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Relying on the stability of etomidate lipid emulsion injection under conditions such as embedding and release. However, so far, the study of materials is still confined to a few systems. Sodium alginate forms salts with divalent metal ions, and the curing of prepared microspheres has been extensively studied for drug delivery release systems. The biocompatibility and degradation behavior of the corrosion of the calcium alginate matrix have a significant impact on drug release and drug delivery systems. In this study, lung-targeting calcium alginate microspheres were prepared by emulsion. The internal and external corrosion behavior of the microspheres was investigated by measuring changes in microsphere size and optical rotation in the dissolution fluid, combined with slices of mouse lung tissue.

Apparatus and materials: AS 780 laser scattering particle size analyzer (U.S. PSS); P-1020 polarimeter (Jasco). Alginate trade name KeltoneLVCR, 0.05Pa·S (1% aqueous solution), molecular weight 54000 (ISP); Na99mTcO4 saline injection.

Received Date: 2004.08.27

Author: Peng Yun (1980), female, graduate, professional direction: particulate preparations. Luwei Yue (1960), male, PhD supervisor, engaged in drug targeting strategies and their agents research. Tel: 021.54237040; Fax: 021.64l78790 E-mail: [email protected] ---- liquid (medicinal, Zhongshan Hospital, Fudan University). Kunming mice (body weight 30~32g, the Court of animals Division).

2 Methods and results

2.1 Calcium alginate blank microsphere preparation using a fixed variable method to study the concentration of sodium alginate, emulsification time, the amount of emulsifier, curing agent concentration, the oil-water ratio, and stirring speed for determining the diameter of calcium alginate blank microspheres. The results show that the optimized process is as follows: take 2.5ml of 40mg/ml sodium alginate aqueous solution, stirred at 900r/min drops into 50ml of rapeseed oil containing 8% Span 80, stirred at room temperature for 1h emulsion, the emulsion slowly trickled mixed solution of acetone containing 0.5CaC1z and ethanol (1:1) for curing 12h. Put it aside, take the precipitate washed with acetone until no droplets at room temperature vacuum drying 12h to get calcium alginate blank microsphere. Obtained microspheres are round, particle size (12.2 ± 8.9) μm.

2.2 In vitro swelling took a small amount of blank microspheres suspended in water for injection and physiological saline, observed under a microscope, the spherical change (see Figure 1), determination of particle size after the swelling of microspheres by swelling ratio = WVo × 100% calculated at time t the volume of the microspheres, the volume of the dry microspheres. The results show that calcium alginate microspheres showed good sphericity in water for injection, particle size in 0.5, 1, 2, 4, and 6h respectively (19.4 Stability of Etomidate Fat Emulsion Injection CHEN Yun-Fa, LU Min, ZHANG Bing (Shanghai Institute of Pharmaceutical Industry, Shanghai 200437)

ABSTRACT: The properties such as size distribution, zeta potential, phase inversion temperature, and drug content of the etomidate fat emulsion injection were examined by dynamic light scattering, electrophoretic light scattering, conductance method, and HPLC, respectively. The results showed that the size distribution and zeta potential of domestic preparation were comparable with the product of Etomidat-Lipuro, the phase inversion temperature was 84°C.

Keywords: etomidate; fat emulsion injection; size distribution; zeta potential; phase inversion temperature

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