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Relying on the stability of etomidate lipid emulsion injection under conditions such as embedding and release, etc. However, so far the study of the material is still confined to a few systems. Sodium alginate forms salts with divalent metal ions, and the curing of the prepared microspheres has been extensively studied as drug release systems. The biocompatibility and degradation corrosion behavior of the calcium alginate matrix has a significant impact on drug release and drug delivery systems. In this study, lung-targeting calcium alginate microspheres were prepared by emulsion. The internal and external corrosion behavior of the microspheres was investigated by measuring changes in the size of the microspheres and the optical rotation of the dissolution fluid, combined with slices of mouse lung tissue.

Apparatus and materials: AS 780 laser scattering particle size analyzer (U.S. PSS); P-1020 polarimeter (Jasco). Alginate trade name KeltoneLVCR, 0.05Pa·S (1% aqueous solution), molecular weight 54000 (ISP); Na99mTcO4 saline injection.

Received Date: 2004.08.27

Author: Peng Yun (1980), female, graduate, professional direction: particulate preparations. Luwei Yue (1960), male, PhD supervisor, engaged in drug targeting strategies and their agents research. Tel: 021.54237040; Fax: 021.64l78790E. mail: [email protected] (Medicinal Liquid, Zhongshan Hospital, Fudan University). Kunming mice (body weight 30~32g, Animal Division).

2 Methods and Results

2.1 Calcium alginate blank microsphere preparation: A fixed variable method was used to study the concentration of sodium alginate, emulsification time, amount of emulsifier, curing agent concentration, oil-water ratio, and stirring speed for determining the ball diameter of calcium alginate blank microspheres. The results show that the optimized process is as follows: Take 2.5ml of 40mg/ml sodium alginate aqueous solution, stir at 900r/min and drop it into 50ml of rapeseed oil containing 8% Span 80. Stir at room temperature for 1 hour to form an emulsion. Slowly trickle the emulsion into a mixed solution of acetone containing 0.5CaC12 and ethanol (1:1) for curing for 12 hours. Set aside, take the precipitate, wash with acetone until no droplets remain, then vacuum dry at room temperature for 12 hours to obtain calcium alginate blank microspheres. The obtained microspheres are round, with a particle size of (12.2 ± 8.9) µm.

2.2 In vitro swelling: A small amount of blank microspheres were suspended in water for injection and physiological saline, observed under a microscope for spherical changes (see Figure 1), and the particle size after swelling was determined by calculating the swelling ratio = WVo × 100% at time t, which represents the volume of the microspheres and the volume of the dry microspheres. The results show that calcium alginate microspheres exhibited good sphericity in water for injection, with particle sizes at 0.5, 1, 2, 4, and 6 hours respectively being (19.4 ± ...

Stability of Etomidate Fat Emulsion Injection CHEN Yun-Fa, LU Min, ZHANG Bing (Shanghai Institute of Pharmaceutical Industry, Shanghai 200437)

ABSTRACT: The properties such as size distribution, zeta potential, phase inversion temperature, and drug content of the etomidate fat emulsion injection were examined by dynamic light scattering, electrophoretic light scattering, conductance method, and HPLC, respectively. The results showed that the size distribution and zeta potential of domestic preparation were comparable with the product of Etomidat-Lipuro, and the phase inversion temperature was 84°C.

Keywords: etomidate; fat emulsion injection; size distribution; zeta potential; phase inversion temperature

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