The positive and negative stereotypes of the ABO blood group do not match. Cause Analysis: Transmission and storage conditions are not applicable, reagent deterioration can lead to failure, resulting in identification errors or with j0 [an error. Experiments should be carried out before quality control reagents for appearance, potency, and other projects. The preparation of standard quality red cells of blood from different anticoagulants in small laboratories should be configured according to the required concentration to correctly configure red blood cells. It is best to purchase commercial reagent red blood cells to ensure the correctness of the test results.
2.1 Red blood cells, red blood cells, and serum itself cause suspension to be too thick or too short, the proportion of antigen-antibody inappropriate, leading to misjudgment of anti-hundred negative reaction.
2.2 Subjects with erythrocyte antigen sites too small (e.g., type) or less antigenic (such as death of Jm disease or malignant neoplasm).
2.3 By the influence of Qing Lan fi white disorder (high globulin m disease) or high temperature experiments, often 0f starting cells showed cord-like money order.
2.4 By the influence of clear lack of some anti-A or anti-B antibodies, such as globulin [J deficiency.
2.5 Various causes red blood cells dissolve, mistaken for agglutination partial melting m, {； = f m-type material and the corresponding antibodies.
2.6 Bacterial contamination or genetic factors jf leading to multi-agglutination or umbrella agglutination.
2.7 Clear accident anti-break, such as their own anti-I, often disturbing stereotypes.
2.8 By l0 who JgJL plasma [J ball of white down the value of the business concentration of fibrinogen and other problems can lead to the formation of string-shaped red blood money, resulting in the phenomenon of false agglutination.
2.9 Infant anti break the water to lower or passive shaped by the mother, antibodies, antibody of water for the elderly decline, J [J abnormal results to the test.
Irregular antibodies and subtype of m-type test foot antigen-antibody reaction, when patients I Sapporo clear deposit outside the ABO type IgM or] of gG and IgM type of irregular antibodies, ABO positive and negative stereotypes are inconsistent. To the hoop to set sail type material in the determination of saliva AB0m type. Due to weak antigen expression, R antigen expression is particularly weak, may [Article ID] 1819-0774 (2008) 10-049-01 counter-stereotypical small match,ugg pas cher, ¨； the jI since stereotypes wrong meeting.
Experiment using the test tube weep, when necessary, the adsorption and elution test to further determine the card common original six the I F ordinary clear the condensation set cable titer is generally high, month .14 °； C to live JI, but due to differences in the human immune system, but can not speak swelling or pathogen Hugh infection _ Ding lI-inch cold anti- autoantibodies can be abnormally high levels, resulting in a bath 10 [Xing poor this cold agglutinin titer 4 °； C higher than 1000, with 37 ℃ saline Shen Di-cell, 37 °； C environment sting operation to exclude ten of the cold agglutinin interference.
Clear put five technical and management errors equipment, can produce false m results; leakage 7JII reagents, resulting in false negatives; record the result or error of judgment or specimen centrifugation speed and the time is right, can produce false positive or false negative results six other factors for certain diseases can lead to the ABO system antigen sad is suppressed, antigen weakened elm flf difficulties; abnormal red blood cell I abnormal or help get performance, cho multi-agglutination phenomenon occurs; m-type-specific substance over high and anti-A or anti-B hole-type reagents, causing the stereotypes of abnormal results; dextran, intravenous contrast agents can cause false agglutination of red blood cells; the near future the people the amount of plasma exchange treatment, clear the specimens m contains the input {lj! donor anti-A or anti-B antibodies cause stereotypes wrong; recently lost j0 [allows m liquid standard Bong become a red blood cell mixture, shaping the mixed appearance of agglutination phenomenon.
Science and a high degree of responsibility can not speak of T as the paint set results side of the to be carefully observed is weak agglutination; particularly foot right, anti-stereotyped characters results in a timely manner the review will infants Increase) to JJJ absorption put open experiments it experimental day; when J strong m liquid standard instructions from the quality management the development of the bilge fluid specimen collection process aspects of the standard operating bilge Cheng and strictly enforced to ensure that the standard Upon acquisition, transport, receive, save, Bo 'effective control during the destruction process, specimens for scientific management, to ensure that the specimen 11 on the heart to avoid the subscript l Upon dilution and dissolved 10L generation to ensure that the m-type results are accurate to talk about further impaired the security umbrella and effectiveness of the clinical lose J0 [.