North Face UK: Several NLVs RNA-dependent RNA polymerase nucleotide sequence analyses. To investigate the expression of Q adhesion molecule 1 (1CAM-1) in thyroid carcinoma and its significance; Immunohistochemical methods (sP France) were used to detect the expression levels of cell adhesion molecule-1 in 40 cases of thyroid cancer and 5 cases of normal thyroid tissue. Computer image analysis techniques were employed to measure the average optical density of cell adhesion molecule-1 expression in different types of thyroid cancer and normal thyroid tissue specimens, as well as the average positive area rate. The vascular endothelial adhesion molecule-1 in the thyroid cancer cell membrane and cytoplasm appeared brown with high expression; cells in normal thyroid tissue specimens did not express adhesion molecule-1. Image analysis showed that the average optical density of 1CAM-1 expression in thyroid cancer was 0.2138, higher than the 0.0759 of normal thyroid tissue. The difference was significant (P < O.O1). Conclusion: 1CAM-1 plays a role in the formation process of the lumen in thyroid cancer and is involved in angiogenesis.
Keywords: thyroid cancer; cell adhesion molecule 1; immunohistochemistry
Thyroid cancer is the most common endocrine malignancy. Tumor growth and metastasis require angiogenesis. The relationship between ICAM-1 and angiogenesis has been rarely reported abroad. It was found that in the early formation of the blood-brain barrier in mice (5 to 7 days after birth), the luminal surface of vascular endothelial cells highly expressed ICAM-1; in mature blood-brain barrier structures (14 days after birth) and adult mouse brains, the cavity surface of vascular endothelial cells greatly weakened or even disappeared ICAM-1 expression, suggesting that ICAM-1 is involved in early angiogenesis. However, ICAM-1 expression in thyroid carcinoma has not been reported. In this study, immunohistochemical methods were used to detect the expression level of cell adhesion molecule-1 in thyroid cancer and normal thyroid tissue specimens, aiming to explore the relationship of ICAM-1 in the process of tumor formation and angiogenesis.
1 Materials and Methods:
1. Material sources collected in our hospital from 2000 to 2004, confirmed by surgery and pathology of thyroid cancer, the specimens were fixed with 10% formalin, paraffin-embedded, HE stained, slice thickness 4μm. Age ranged from 20 to 60 years old, male: female ratio 1:1.6, average age 35.6 years, no preoperative adjuvant therapy was performed. Another 5 cases of normal thyroid tissue were used as control.
1.2 Reagents: S-P Universal immune histochemistry kit, product of Santa Cruz company, USA; concentrated mouse anti-human ICAM-1 monoclonal antibody, product of Zymed, USA; DAB color reagent and polylysine from Sigma, USA. The above reagents were purchased from Beijing Zhongshan Biotechnology Co., Ltd.. Prepared distribution 0.3H2O2 0.01MPBS (pH7.4), 0.01M citric acid buffer.
1.3 Streptavidin anti-biotin peroxidase enzymatic (streptavidinproxidasemethod, S-P method) main steps: Conventional dewaxing of paraffin slices, ethanol hydration, 0.3HzO: solution for 10 minutes to inhibit endogenous oxidases, microwave repair.
Comparative Analysis of Nucleotide Sequence of NLVs RNA-dependent RNA Polymerase Zhang Lun, et al (Department of Basic Sciences, Beihua University, Jilin 132001)
Abstract Quantitative analysis of nucleotide sequence of 5 NLVs RNA-dependent RNA polymerase with Yuan Jian method. CR840, Hawii, and Lord have high autoploidy. CR840, NV, and KY a 89 have low autoploidy. This method lays a foundation for quantitative analysis of RNA nucleotide sequence.
Keywords: Yuan Jian: of NIV Received date: 2005-04-26 Corresponding author: Duan-Lian Zhang #, Wuhan University School of Medicine * Wuhan University School of Medicine, Master Organization embryos Department ·； 554 ·；