Different processing conditions for pregnant women with IgG A (B) antibody determination influence the results. Tube mouth plugged, 37°C water bath for 2 hours, after diluting the processing liquid ratio and adding it to micro-column gel holes, each hole containing 25L, along with the corresponding 0.8% A, B standard red cells 501 ~ 1, moncler jacken, incubated at 37°C for 15 minutes, centrifuged for 10 minutes, results in the appearance of "+" as positive. Serum 0.4ml mixed with Me0.4ml in a 37°C bath for 30 minutes, LH, 1.5 hours, 2.5 hours, 4 hours, detected according to the above steps, do 30 minutes, LH, 1.5 hour titer only when measuring > 2H when the measured value L 3 titer (as 2H aging is the price of 64 only measured 128, 256, 512), in 2.5 hours, 4 hours only when < 2H L 2 titer.
Results: Among 36 cases, 2 specimens had an Me 2H potency: < l:64 in 2L cases, 8 cases of L:64, l:128 in 4 cases, 2 cases of L:256, l:512 in 0 cases reported in the literature, such as the L:64 when there is a risk of ABO-HDN. We consider more than l:64 as positive... Then, the positive rate was 41.7% (15/36), slightly higher than the conventional in vitro assay reported positive rate (13.6%-33.1%), such as l:128 as positive, the positive rate was 19.4% and reported in the literature with micro column gel method sensitivity higher than the tube method L 2 titer. Furthermore, the results of 2H are consistent with the conventional, can be used as a standard time. In 2.36 cases by 30 minutes, LH, 1.5 hours, 2.5 hours, 4 hours after treatment with IgG of A (B) test results are shown in Table L. Table L displays that with 2 Me treatment extended the time, IgG A (B) detection results with standard time 2 hours, 30 minutes were positive in 36 cases, the positive rate of 100%, and 2hBvJ" the positive rate was 41.7%, X2 = 26.89, P<0.001, indicating that the 30 minutes were significantly different from 2H, IgM antibodies are not completely destroyed, 30 minutes cannot be used as the detection time, LH results with 2H are slightly different, but in order to ensure result accuracy we will exclude, 1.5-4 hours results without any changes.
Discussion: ABOHDN is due to maternal-fetal blood group incompatibility, maternally derived IgG of A (B) antibody invades the placenta into fetal circulation, packages and destroys the fetal red blood cells, causing hemolysis, so in order to prevent and avoid the occurrence of HDN, on pregnancy women in addition to conventional prenatal examination, O type of pregnant women should undergo antibody screening. The incidence of HDN increases with increasing levels of maternal anti-A (B) antibody, thus accurate detection of maternal serum presence or absence of IgG nature of the antibody and potency can predict the possible occurrence of ABOHDN. O type anti-A, anti-B is often a mixture of Igg and IgM, they have the same specificity, to separately detect Igg of A (B) must remove IgM of A (B) interference, 2 Me IgM cracking, but cannot inactivate IgG antibody, literature often uses treatment 2H determination. But sometimes limited by conditions, various laboratory processing times are short, so understanding the effect of different treatment times on experimental results is very necessary.
After the experiment, from Table L results we can know, in the 30 minutes when the measured results higher than 2H L 2 titer, significant differences still exist, serum IgM antibody is not destroyed, this time is not suitable for detecting. Although LH has some little differences, not significantly different, but in order to ensure the accuracy of the results, also cannot be used to detect, 1.5 hours after the test results are the same as 2 hours. And as time changes, antibody does not change to maintain a certain stability, which explains that 2 Me on type Igg antibodies without damage, is a relatively ideal treatment reagent.