The detection and analysis of serum myocardial zymogram in children with rotavirus enteritis showed clear increases in myocardial enzyme activity, indicating that rotavirus infection can cause myocardial injury. Additionally, varying degrees of dehydration were observed in the RV enteritis group. The moderate dehydration group exhibited significantly increased serum myocardial enzyme activity, suggesting that the degree of myocardial injury is closely related to the severity of the disease. Based on this information, serum myocardial enzyme activity can be used as an important index for monitoring the condition and prognosis of children with rotavirus enteritis. During treatment, vitamin C, coenzyme Q, and energy mixtures can be used to assist antiviral therapy and improve blood circulation. Timely correction of dehydration, acidosis, and electrolyte disorders are also important measures to improve myocardial injury caused by rotavirus infection. In clinical practice, myocardial injury in children with rotavirus enteritis often presents with mild symptoms and can easily be overlooked. Therefore, early screening of myocardial enzymes in children is necessary for early diagnosis and timely, accurate treatment.