From "Protein Electrophoresis Laboratory Techniques"

by kfjro549 on 2011-05-31 10:54:02

Guangzhou LiHao Health & Beauty Information - The Choice of Protease Inhibitors

In 2008, at the beginning of the year, summaries of various cosmetics were overwhelming. The Biotech website will also present a grand feast of biotechnology in the coming months, providing readers with cutting-edge technology and the latest information for intellectual enjoyment. Picture-in-picture advertisements begin.

Picture-in-picture advertisements end. Every day, carefully selected articles from manufacturers and distributors about key products promoted in mainland China over the past year or newly launched products and technologies are presented. This presents the products and technologies that were highly sought after on the Biotech website in 2007, creating a "visual feast" of biotechnology products.

When cells are crushed, finally pour in almonds. Cosmetic weight loss, proteolytic enzymes will be released or activated, complicating the electrophoresis results and affecting the final analysis. To avoid these situations, it is recommended to carry out sample preparation as much as possible under low-temperature conditions. Moreover, many proteases become inactive above pH 9, so Tris base, sodium carbonate, or basic carrier ampholytes often inhibit proteolysis. However, some proteases remain active under these conditions, and in such cases, protease inhibitors should be used. Therefore, for the proteins we want to study, selectively and effectively inhibiting these proteases is particularly important for protein purification route design.

Protease inhibitors, like proteases, are complex and diverse. Many chemicals are commonly used as protease inhibitors, such as PMSF and leupeptin. Since one protease inhibitor only works on a specific class of proteases, it is usually recommended to use a combination of protease inhibitors, such as broad-spectrum protease inhibitor "cocktail," see the table below (from "Electrophoresis Techniques for Proteins").

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